Cholesterol Oxidase Antibody Biotin Conjugated

SKU: orb344304

Description

Cholesterol Oxidase antibody (Biotin)

Images & Validation

• Tested Applications: DOT, ELISA, IP, WB
• Dilution Range: ELISA: 1:5,000 - 1:20,000, IP: 1:100, WB: 1:500 - 1:5,000
• Reactivity: Bacteria
• Application Notes:
  Anti-Cholesterol Oxidase has been tested by ELISA, dot blot, and western blot. This product is assayed against 1.0 ug of Cholesterol Oxidase in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product. Reconstitution Buffer: Restore with deionized water (or equivalent). Reconstitution Volume: 100 µL

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Cholesterol Oxidase [Streptomyces]
• Purity: Anti-Cholesterol Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Cholesterol Oxidase [Microorganism]. Cross reactivity against Cholesterol Oxidase from other sources is unknown.
• Conjugation: Biotin

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

goat anti-Cholesterol Oxidase Antibody biotin Conjugation, biotin Conjugated goat anti-Cholesterol Oxidase Antibody, choA antibody, choD antibody, choM antibody, choS antibody, COD antibody

Biorbyt Goat anti Cholesterol oxidase antibody was used to detect purified cholesterol oxidase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified cholesterol oxidase contained 4% BME and were boiled for 5 minutes. Samples of ~1 ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with a 1:3000 dilution of primary antibody (ON 4 C in orb348637). Detection shown was using Dylight 488 conjugated Donkey anti goat (1:10K in TBS/orb348637 1 hr RT).

Quick Database Links

UniProt

P12676