Chk2 (phospho Ser516) rabbit pAb

• Catalog Number: orb764162
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Chk2 (phospho-Ser516)
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Monkey
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Immunogen: The antiserum was produced against synthesized peptide derived from human Chk2 around the phosphorylation site of Ser516. AA range:486-535
• MW: 61
• Tested applications: ELISA, WB
• Dilution range: Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-CHEK2 antibody, anti-CDS1 antibody, anti-CHK2
• Note: For research use only

Western Blot analysis of various cells using Phospho-Chk2 (S516) Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Western blot analysis of lysates from HeLa cells treated with UV, using Chk2 (Phospho-Ser516) Antibody. The lane on the right is blocked with the phospho peptide.