Chk1 (phospho Ser286) rabbit pAb

• Catalog Number: orb767469
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Chk1 (phospho-Ser286)
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Immunogen: The antiserum was produced against synthesized peptide derived from human Chk1 around the phosphorylation site of Ser286. AA range:256-305
• MW: 55
• Tested applications: ELISA, IF, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-CHEK1 antibody, anti-CHK1 antibody, anti-Seri
• Note: For research use only

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Chk1 (Phospho-Ser286) Antibody.

Immunofluorescence analysis of HeLa cells, using Chk1 (Phospho-Ser286) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from K562 cells treated with Na3VO4 0.3uM 40', using Chk1 (Phospho-Ser286) Antibody. The lane on the right is blocked with the phospho peptide.