Chicken IgM (mu chain) Antibody Fluorescein Conjugated

SKU: orb346888

Description

Chicken IgM (mu chain) antibody (FITC)

Images & Validation

• Tested Applications: FC, FLISA, IF
• Dilution range: FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000
• Reactivity: Gallus
• Application Notes:
  This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Key Properties

• Antibody Type: Secondary Antibody
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Chicken IgM whole molecule
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Chicken IgM and Chicken Serum. No reaction was observed against other chicken heavy or light chain proteins.
• Conjugation: FITC

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 1.0 mg/mL
• Disclaimer: For research use only

Alternative Names

goat anti-Chicken IgM (mu chain) Antibody Fluorescein Conjugation, goat anti-Chicken IgM mu chain FITC Conjugated Antibody

Characterization of bursal B-lymphocyte (BBL) cultures. Cell viability of BBLs was determined by the trypan blue exclusion method over 120 min. (C), mature B cells (α-IgG) (D), and immature B-cells (α-IgM) (E). DAPI staining was used to detect cell nuclei. Negative controls were prepared in the absence of primary antibodies (F–H).