Chicken IgG (H&L) Antibody Biotin Conjugated Pre-Adsorbed

• Catalog Number: orb346930
• Category: Antibodies
• Description: Chicken IgG (H&L) antibody (Biotin)
• Clonality: Polyclonal
• Species/Host: Donkey
• Isotype: IgG
• Conjugation: Biotin
• Reactivity: Gallus
• Form/Appearance: Lyophilized
• Concentration: 1.0 mg/mL
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads followed by conjugation and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Donkey Serum, Chicken IgG and Chicken Serum. No reaction was observed against Bovine, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit, Rat and Sheep Serum Proteins. This antibody will react with heavy chains of Chicken IgG and with light chains of most Chicken immunoglobulins.
• Immunogen: Chicken IgG whole molecule
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:10,000 - 1:50,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Application notes: Anti-Chicken IgG Biotin Antibody has been tested by ELISA and is suitable for ELISA, Western Blot and Immunohistochemistry applications. Specific conditions for reactivity should be optimized by end user.
• Antibody Type: Secondary Antibody
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Alternative names: Donkey anti-Chicken IgG Antibody Biotin Conjugated
• Note: For research use only

Western Blot of Unconjugated Anti-Chicken IgG (H&L) (DONKEY) Antibody (Min X Bv Gt GP Ham Hs Hu Ms Rb Rt & Sh Serum Proteins) (p/n orb346926). Lane M: 3 µl Molecular Ladder. Lane 1: Chicken IgG whole molecule (p/n orb2652774). Lane 2: Chicken IgG F(c) Fragment (p/n orb346051). Lane 3: Chicken IgG Fab Fragment (p/n orb2652771). Lane 4: Chicken IgM Whole Molecule (p/n orb346054). Lane 5: Chicken Serum. All samples were reduced. Load: 50 ng per lane. Block: orb348637 for 30 min at RT. Primary Antibody: Anti-Chicken IgG (H&L) (DONKEY) Antibody (Min X Bv Gt GP Ham Hs Hu Ms Rb Rt & Sh Serum Proteins) (p/n orb346926) 1:3000 for 60 min at RT. Secondary antibody: Anti-Donkey IgG (GOAT) Peroxidase Conjugated Antibody (p/n orb347994) 1:40000 in orb348637 for 30 min at RT. Predicted/Observed Size: 25 and 72 kDa for Chicken IgY and Serum, 25 kDa for F(c) and Fab, 75 kDa for IgM. Chicken F(c) migrates slightly higher.