Chicken IgG (H&L) Antibody Biotin Conjugated

• Catalog Number: orb346896
• Category: Antibodies
• Description: Chicken IgG (H&L) antibody (Biotin)
• Clonality: Polyclonal
• Species/Host: Goat
• Isotype: IgG
• Conjugation: Biotin
• Reactivity: Gallus
• Form/Appearance: Lyophilized
• Concentration: 2.0 mg/mL
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Purity: Anti-Chicken IgG antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Chicken IgG and Chicken Serum.
• Immunogen: Chicken IgG whole molecule
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:98,500, IHC: 1:500 - 1:3,000, WB: 1:3,000 - 1:30,000
• Application notes: Anti-Chicken IgG Biotin conjugated antibody has been tested by ELISA and is suitable for ELISA, Western Blot and Immunohistochemistry applications. Specific conditions for reactivity should be optimized by end user.
• Antibody Type: Secondary Antibody
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Alternative names: goat anti-Chicken IgG Antibody Biotin Conjugation,
• Note: For research use only

ELISA results of purified Goat anti-Chicken IgG Biotin Conjugated Antibody tested against purified Chicken IgG (p/n orb2652774). Each well was coated in duplicate with 1.0 µg of Chicken IgY (blue line). The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer, Streptavidin HRP conjugate.