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Catalog Number | orb346906 |
---|---|
Category | Antibodies |
Description | Chicken IgG F(c) antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | DOT, ELISA, IHC, WB |
Reactivity | Gallus |
Isotype | IgG |
Immunogen | Chicken IgG F(c) fragment |
Concentration | 2.1 mg/mL |
Dilution range | ELISA: 1:20,000 - 1:100,000, IHC: 1:1000-1:5000, WB: 1:2000-1:10,000 |
Form/Appearance | Liquid (sterile filtered) |
Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Chicken IgG, Chicken IgG F(c) and Chicken Serum. No reaction was observed against Chicken IgG F(ab). |
Conjugation | Unconjugated |
Storage | Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | rabbit anti Chicken IgG F(c) Antibody, rabbit anti Read more... |
Note | For research use only |
Application notes | Anti-Chicken IgG F(c) antibody has been tested by LEISA, dot blot, and western blot and is suitable for immunohistochemistry, as well as other assays requiring lot-to-lot consistency. |
Expiration Date | 12 months from date of receipt. |
SDS-PAGE | |
Unconjugated | |
This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and papain digestion followed by chromatographic separation and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken Serum, anti-Chicken IgG and anti-Chicken IgG F(c). No reaction was observed against anti-Chicken IgG F(ab’)2 or anti-Papain. | |
Chicken |
SDS-PAGE | |
FITC | |
This product was prepared from normal serum by delipidation, salt fractionation and ion change chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Chicken IgG, anti-Chicken IgG F(c) and anti-Chicken Serum. No reaction was observed against anti-Chicken IgG F(ab’)2 or anti-Papain. | |
Chicken |
SDS-PAGE | |
HRP | |
This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti- Chicken IgG, anti-Chicken IgG F(c) and anti-Chicken Serum. No reaction was observed against anti-Chicken IgG F(ab’)2 or anti-Papain. | |
Chicken |
SDS-PAGE | |
Biotin | |
This product was prepared from normal serum delipidation, salt fractionation, ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Chicken IgG, anti-Chicken IgG F(c) and anti-Chicken Serum. No reaction was observed against anti-Chicken IgG F(ab’)2 or anti-Papain. | |
Chicken |
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