CHD4 Antibody / Chromodomain helicase DNA binding protein 4

SKU: orb639584

Description

CHD4/Mi-2b is a component of the nucleosome remodeling and deacetylase (NuRD) complex which is a multi-subunit protein complex containing both histone deacetylase and nucleosome-dependent ATPase subunits. Current models predict that this complex function primarily in transcriptional repression. Accumulating evidence indicates that NuRD may regulate the transcription of specific genes by interacting with specific transcriptional factors. In addition, the NuRD complex may also participate in genome-wide transcriptional regulation through an association with histone tails.

Images & Validation

• Tested Applications: ELISA, IF, IHC-P, WB
• Dilution range: ELISA (order BSA-free format for coating),Western blot: 1-2ug/ml,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Reactivity: Human, Mouse
• Application Notes:
  Optimal dilution of the CHD4 antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2a, kappa
• Clone No.: 3F2/4
• Immunogen: Amino acids ASGLGSPSPCSAGSEEEDM and CSRLANRAPEPPPQQVAQQQ were used as the immunogen for the CHD4 antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

Immunofluorescent staining of permeabilized human MCF7 cells with CHD4 antibody (clone 3F2/4, green) and Phalloidin (red).

IHC staining of FFPE human breast carcinoma with CHD4 antibody (clone 3F2/4). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

Western blot testing of human SK-BR-3 cells with CHD4 antibody. Predicted molecular weight ~260 kDa.

SDS-PAGE analysis of purified, BSA-free CHD4 antibody (clone 3F2/4) as confirmation of integrity and purity.

Quick Database Links

UniProt

Q14839