CHD1 Antibody, KO Validated

• Catalog Number: orb1274419
• Category: Antibodies
• Description: CHD1 Antibody, KO Validated
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1600 to the C-terminus of human CHD1 (NP_001261.2).
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 197kDa
• Target: CHD1
• UniProt ID: O14646
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: CHD1, chromodomain helicase DNA binding protein 1,
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:100 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using CHD1 Antibody (orb1274419) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Western blot analysis of extracts from normal (control) and CHD1 knockout (KO) 293T cells, using CHD1 antibody (orb1274419) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded mouse brain using CHD1 antibody (orb1274419) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human liver damage using CHD1 antibody (orb1274419) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat brain using CHD1 antibody (orb1274419) at dilution of 1:100 (40x lens).