cGKI (cGKI beta) Antibody (C-term)

• Catalog Number: orb1939472
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Target: This cGKI (cGKI beta) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 629-660 amino acids from the C-terminal region of human cGKI beta.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: Q13976
• MW: 76364 Da
• Tested applications: IHC-P, WB
• Dilution range: WB: 1:1000, WB: 1:1000, IHC-P: 1:100, IHC-P: 1:100
• Clone Number: RB1046
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: cGMP-dependent protein kinase 1, cGK 1, cGK1, cGMP
• Note: For research use only
• NCBI: NP_006249.1, NP_001091982.1

All lanes: Anti-cGKI (cGKI beta) Antibody (C-term) at 1:1000 dilution. Lane 1: 293 whole cell lysate. Lane 2: Hela whole cell lysate. Lane 3: U-87 MG whole cell lysate. Lane 4: Mouse small intestine tissue lysate. Lane 5: NIH/3T3 whole cell lysate. Lane 6: C6 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 76 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-cGKI (cGKI beta) Antibody (C-term) at 1:1000 dilution + SH-SY5Y whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 76 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemical analysis on paraffin-embedded Human hepato carcinoma tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary Antibody.

Immunohistochemical analysis on paraffin-embedded Human placenta tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary Antibody.