CFTR Antibody / Cystic Fibrosis Transmembrane Regulator

• Catalog Number: orb606381
• Category: Antibodies
• Description: Cystic fibrosis transmembrane conductance regulator (CFTR) is a membrane protein and chloride channel in vertebrates that is encoded by the CFTR gene. The CFTR gene codes for an ABC transporter-class ion channel protein that conducts chloride and thiocyanate ions across epithelial cell membranes. Mutations of the CFTR gene affecting chloride ion channel function lead to dysregulation of epithelial fluid transport in the lung, pancreas and other organs, resulting in cystic fibrosis. Complications include thickened mucus in the lungs with frequent respiratory infections, and pancreatic insufficiency giving rise to malnutrition and diabetes. These conditions lead to chronic disability and reduced life expectancy. In male patients, the progressive obstruction and destruction of the developing vas deferens (spermatic cord) and epididymis appear to result from abnormal intraluminal secretions, causing congenital absence of the vas deferens and male infertility. [Wiki]
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: CFTR/2290R
• Tested applications: IHC-P
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG, kappa
• Immunogen: A recombinant human partial protein was used as the immunogen for this recombinant CFTR antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
• Purity: Protein A affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This recombinant CFTR antibody is available for research use only.
• Entrez: 1080
• UniProt ID: P13569
• Storage: Store the recombinant CFTR antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the recombinant CFTR antibody should be determined by the researcher. 1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human pancreas with recombinant CFTR antibody (clone CFTR/2290R). HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH9 for 10-20 min followed by cooling at RT for 20 min.

SDS-PAGE analysis of purified, BSA-free recombinant CFTR antibody (clone CFTR/2290R) as confirmation of integrity and purity.