CFL2 Antibody (C-term)

SKU: orb1927130

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Musculoskeletal & Connective Tissue Research

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: IF - 1:25, WB - 1:1000
• Reactivity: Human, Mouse
• Predicted Reactivity: Other, Porcine

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD60821
• Immunogen: This CFL2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 130-144 amino acids from the C-terminal region of human CFL2.
• Target: CFL2
• Molecular Weight: 18737 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Cofilin-2, Cofilin, muscle isoform, CFL2

Western blot analysis of lysates from human skeletal muscle and mouse brain tissue lysate (from left to right), using CFL2 Antibody (C-term). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35 ug per lane.

Fluorescent image of Hela cells stained with CFL2 Antibody (C-term). Diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).

Quick Database Links

Gene Symbol

CFL2

UniProt

Q9Y281