CFL1 Antibody

SKU: orb22812

Description

Rabbit polyclonal antibody to CFL1. The protein is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus. It controls reversibly actin polymerization and depolymerization in a pH-sensitive manner, and is the key component of intranuclear and cytoplasmic actin rods. Additionally, it is widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin.

Images & Validation

• Application Notes:
  Applications: CFL1 antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1,000 ~ 2,000.Recommended starting dilution is 1:1,000

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1 heavy chain and κ light chain
• Clone No.: PAT1C1AT
• Immunogen: Anti-human CFL1 mAb is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human CFL1 amino acids 1-166 purified from E. coli
• Purification: CFL1 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CFL-1, CFL1, Cofilin1, Cofilin-1, Cofilin non-muscle isoform, 18 kDa phosphoprotein, p18.