CFL1 Antibody

SKU: orb1258787

Description

CFL1 Antibody

Research Area

Cancer Biology, Cell Biology, Immunology & Inflammation, Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CFL1 (NP_005498.1).
• Target: CFL1
• Molecular Weight: Observed: 18kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CFL1, 18 kDa phosphoprotein, Cofilin, CFL, Cofilin 1 (non-muscle), Cofilin, non-muscle isoform, p18

Western blot analysis of extracts of various cell lines, using CFL1 antibody (orb1258787) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Immunohistochemistry of paraffin-embedded human appendix using CFL1 antibody (orb1258787) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of NIH/3T3 cells using CFL1 antibody (orb1258787) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC12 cells using CFL1 antibody (orb1258787) at dilution of 1:100. Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

CFL1

UniProt

P23528