Ceruloplasmin Rabbit Polyclonal Antibody

SKU: orb4892

Description

Ceruloplasmin Rabbit Polyclonal Antibody

Research Area

Cell Biology, Cell Markers, Fatty Acid Oxidation, Fatty Acids, Lipid Metabolism, Lipids and Lipoproteins Metabolism, Metabolism, Mitochondrial, Mitochondrial Markers

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Canine, Rabbit

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Ceruloplasmin (551-650/1065aa)
• Target: CP
• Molecular Weight: 120 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CER; caeruloplasmin; Ceruloplasmin precursor; CP 2; CP; CP2; CP-2; EC 1.16.3.1; Ferroxidase; CERU_HUMAN.

Paraformaldehyde-fixed, paraffin embedded (human liver tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Ceruloplasmin) Polyclonal Antibody, Unconjugated (orb4892) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Mouse Serum tissue lysates, Lane 2: Rat Serum tissue lysates, Primary: Anti-Ceruloplasmin (orb4892) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 120 kDa, Observed band size: 140 kDa.

Quick Database Links

Gene Symbol

CP

UniProt

P00450