CEPT1 Antibody (N-term)

• Catalog Number: orb1939020
• Category: Antibodies
• Description: CEPT1 Antibody (N-term)
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Xenopus
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: Q9Y6K0
• MW: 46554 Da
• Tested applications: IHC-P, WB
• Dilution range: WB - 1:1000, IHC-P - 1:100-500
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Research Area: Metabolism Research, Signal Transduction
• Note: For research use only

All lanes: Anti-CEPT1 Antibody (N-term) at 1:2000 dilution. Lane 1: HL-60 whole cell lysate. Lane 2: Human cerebellum tissue lysate. Lane 3: SK-BR-3 whole cell lysate. Lane 4: Human placenta tissue lysate. Lane 5: Mouse spleen tissue lysate. Lane 6: Mouse brain tissue lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 47 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-CEPT1 Antibody (N-term) at 1:4000 dilution. Lane 1: HL-60 whole cell lysate. Lane 2: Hela whole cell lysate. Lane 3: Human cerebellum tissue lysate. Lane 4: SK-BR-3 whole cell lysate. Lane 5: Mouse brain tissue lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 47 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-CEPT1 Antibody (N-term) at 1:1000 dilution + Hela whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 47 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemical analysis of paraffin-embedded Human breast tissue performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary Antibody.

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary Antibody.