CENPA Antibody (Biotin)

SKU: orb148110

Description

Mouse monoclonal to CENP-A (Biotin). A replicated chromosome includes two kinetochores that control chromosome segregation during mitosis. The Centromere Protein-A, CENP-A, is a Histone H3-like protein that contains a C-terminal H3-like domain, which is required for centromere localization of CENP-A, and an antigenic N-terminal domain. CENP-A, originally isolated from HeLa cells, is essential for kinetochore targeting of CENP-C. In the presence of DNA CENP-A forms an octa-meric complex with histones H4, H2A, H2B. CENP-A specifically localizes to active centromeres and is a component of specialized centromeric nucleosomes, on which kinetochores are assembled. CENP-A is essential for nucleosomal packaging of centromeric DNA at interphase and functions as a centromere formation marker on the chromosome..

Research Area

Epigenetics & Chromatin

Images & Validation

• Tested Applications: ICC, IF, WB
• Dilution Range: WB (1:1000), ICC/IF (1:100)
• Reactivity: Human
• Application Notes:
  1 µg/ml was sufficient for detection of CENPA in 20 µg of U2OS cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: 5A7-2E11
• Immunogen: Synthetic peptide corresponding to a portion of human CENP-A
• Target: CENPA
• Molecular Weight: 18kDa
• Purification: Protein G Purified
• Conjugation: Biotin

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 136.36mM Ethanolamine, 133.23 mM Chlorides, 9.55mM Phosphates, 9.55mM Sodium Bicarbonate
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CENPA, CENP-A, Centromere autoantigen A, Histone H3-like centromeric protein A

Quick Database Links

Gene Symbol

CENPA

UniProt

P49450

NCBI Gene ID

1058

RefSeq (Protein):NP_001035891.1