CENP-A Antibody: Biotin

• Catalog Number: orb148110
• Category: Antibodies
• Description: Mouse monoclonal to CENP-A (Biotin). A replicated chromosome includes two kinetochores that control chromosome segregation during mitosis. The Centromere Protein-A, CENP-A, is a Histone H3-like protein that contains a C-terminal H3-like domain, which is required for centromere localization of CENP-A, and an antigenic N-terminal domain. CENP-A, originally isolated from HeLa cells, is essential for kinetochore targeting of CENP-C. In the presence of DNA CENP-A forms an octa-meric complex with histones H4, H2A, H2B. CENP-A specifically localizes to active centromeres and is a component of specialized centromeric nucleosomes, on which kinetochores are assembled. CENP-A is essential for nucleosomal packaging of centromeric DNA at interphase and functions as a centromere formation marker on the chromosome..
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 5A7-2E11
• Tested applications: ELISA, ICC, IF, IHC, WB
• Reactivity: Human
• Isotype: IgG1
• Immunogen: Synthetic peptide corresponding to a portion of human CENP-A
• Concentration: 1 mg/ml
• Dilution range: WB (1:1000), ICC/IF (1:100)
• Conjugation: Biotin
• MW: 18kDa
• Target: CENPA
• Entrez: 1058
• UniProt ID: P49450
• NCBI: NP_001035891.1
• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 136.36mM Ethanolamine, and 9.55mM Sodium Bicarbonate in 95.45% PBS
• Alternative names: CENP A antibody, cenpa antibody, Centromere auto a
• Note: For research use only
• Application notes: 1 µg/ml of SMC-202 was sufficient for detection of CENPA in 20 µg of U2OS cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Expiration Date: 12 months from date of receipt.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-CENP-A Monoclonal Antibody, Clone 5A7-2E11. Tissue: Colon cancer cell line (HT-29). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-CENP-A Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus. Magnification: 60X.

Western Blot analysis of Human U2OS cell lysate showing detection of CENP-A protein using Mouse Anti-CENP-A Monoclonal Antibody, Clone 5A7-2E11. Primary Antibody: Mouse Anti-CENP-A Monoclonal Antibody at 1:1000.