Cellular Apoptosis Susceptibility Antibody (C-term)

SKU: orb1932372

Description

Cellular Apoptosis Susceptibility Antibody (C-term)

Research Area

Cell Biology, Signal Transduction

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Dilution Range: IF - 1:100, IHC-P - 1:100, WB - 1:1000
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Zebrafish

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Molecular Weight: 110417 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Disclaimer: For research use only

Alternative Names

CAS {ECO:0000303|PubMed:7479798}, XPO2

Immunohistochemical analysis of paraffin-embedded H. tonsil section using CSE1L Antibody. Diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded H. stomach section using CSE1L Antibody. Diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Fluorescent image of HeLa cells stained with Cellular Apoptosis Susceptibility Antibody (N-term). Diluted at 1:100 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).

Western blot analysis of anti-CSE1L Pab in, from left to right, A375, CEM, and mouse heart cell line lysates (35 ug/lane). CSE1L (arrow) was detected using the purified Pab.

Western blot analysis of lysates from Hela, Jurkat, Ramos, mouse NIH/3T3 cell line (from left to right), using CSE1L Antibody. Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35 ug per lane.

Quick Database Links

UniProt

P55060