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Catalog Number | orb767362 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to C/EBP alpha (phospho-Thr226) |
Clonality | Polyclonal |
Isotype | IgG |
Conjugation | Unconjugated |
Reactivity | Human, Monkey, Mouse, Rat |
Concentration | 1 mg/ml |
Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Immunogen | The antiserum was produced against synthesized peptide derived from human C/EBP-alpha around the phosphorylation site of Thr226. AA range:192-241 |
MW | 37 |
Tested applications | ELISA, WB |
Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
Source | Rabbit |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | Anti-CEBPA antibody, anti-CCAAT/enhancer-binding p Read more... |
Note | For research use only |
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using C/EBP-alpha (Phospho-Thr226) Antibody.
Western blot analysis of lysates from COS7 cells treated with EGF 200ng/ml 30', using C/EBP-alpha (Phospho-Thr226) Antibody. The lane on the right is blocked with the phospho peptide.