CEBP Delta antibody

• Catalog Number: orb345611
• Category: Antibodies
• Description: CEBP Delta antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, IP, WB
• Reactivity: Mouse
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to the amino terminus of mouse C/EBP.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:4,000 – 1:9,000, IHC: 1:2000, IP: 0.8 – 1.5 µg/ml, WB: 1:1,000 - 1:15,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody recognizes endogenous mouse and recombinant human C/EBP delta protein. A BLAST analysis was used to suggest cross-reactivity with C/EBPd from human sources based on 77% homology with the immunizing sequence. No cross-reactivity is observed with mouse C/EBPa or C/EBPb. Reactivity with C/EBPd from other sources has not been determined.
• Conjugation: Unconjugated
• UniProt ID: Q00322
• NCBI: 110347410
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-CEBP Delta Antibody, C/EBP related pro
• Note: For research use only
• Application notes: This affinity purified antibody has been tested for use in ELISA, western blotting, and suitable in IHC. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 35 kDa in size corresponding to C/EBPd by western blotting in the appropriate cell lysate or extract. The predicted molecular weight of mouse C/EBPd is 28.8 kDa. This antibody is capable of detecting both over-expressed and endogenous C/EBPd.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Lane 1: untreated wild-type (WT) cells Lane 2: WT cells treated 3 h with LPS Lane 3: C/EBPd-knock-out cells treated 3H with LPS Load: 35 ug per lane using CEBP Delta antibody

Western blot analysis of Lane 1: human C/EBPbeta Lane 2: Mouse C/EBPdelta Lane 3: untransfected Lane 4: human C/EBPdelta Load: 35 ug per lane using CEBP Delta antibody