CDX2 Antibody

SKU: orb750063

Description

The intestine-specific transcription factors CDX1 and CDX2 are important for directing intestinal development, differentiation, proliferation and maintenance of the intestinal phenotype. CDX2 protein expression has been seen in GI carcinomas. Anti-CDX2 has been useful to establish GI origin of metastatic adenocarcinomas and carcinoids and is especially useful to distinguish metastatic colorectal adenocarcinoma from lung adenocarcinoma. However, mucinous carcinomas of the ovary also express CDX2 protein. It limits the usefulness of this marker in the distinction of metastatic colorectal adenocarcinoma from mucinous carcinoma of the ovary.

Research Area

Developmental Biology, Epigenetics

Images & Validation

• Tested Applications: ELISA, IHC-P
• Dilution range: ELISA: 2-4ug/ml (order BSA/azide-free format),Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Reactivity: Human
• Application Notes:
  The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CDX2 antibody to be titered up or down for optimal performance.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2a, kappa
• Clone No.: CDX2/1690
• Immunogen: Amino acids 150-249 from the human protein were used as the immunogen for this CDX2 antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 1X PBS, pH 7.4
• Disclaimer: For research use only

IHC testing of FFPE human colon carcinoma with CDX2 antibody (clone CDX2/1690). Required HIER: boil sections in 10mM citrate buffer, pH6, for 10-20 min.

IHC testing of FFPE human colon carcinoma with CDX2 antibody (clone CDX2/1690). Required HIER: boil sections in 10mM citrate buffer, pH6, for 10-20 min.

SDS-PAGE Analysis of Purified, BSA-Free CDX2 Antibody (clone CDX2/1690). Confirmation of Integrity and Purity of the Antibody.

Quick Database Links

UniProt

Q99626