CDKN3 Antibody (C-term)

• Catalog Number: orb1929184
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Target: This CDKN3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 183-212 amino acids from the C-terminal region of human CDKN3.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• UniProt ID: Q16667
• MW: 23805 Da
• Tested applications: IHC-P, WB
• Dilution range: WB: 1:2000, IHC-P: 1:50~100
• Antibody Type: Primary Antibody
• Clone Number: RB3225
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Cyclin-dependent kinase inhibitor 3, CDK2-associat
• Research Area: Cancer Biology, Cell Biology, Signal Transduction
• Note: For research use only
• NCBI: NP_001124323.1, NP_005183.2
• Expiration Date: 12 months from date of receipt.

Anti-hCDKN3-A198 at 1:2000 dilution + HL-60 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 24 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.