CDKN1C Antibody (N-term)

SKU: orb1938584

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cancer Biology, Cell Biology

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: IF - 1:10-50, WB - 1:1000
• Reactivity: Human, Mouse

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD31269
• Immunogen: This CDKN1C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 34-64 amino acids from the N-terminal region of human CDKN1C. Antigen Region: 34-64 aa.
• Target: CDKN1C
• Molecular Weight: 32177 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Cyclin-dependent kinase inhibitor 1C, Cyclin-dependent kinase inhibitor p57, p57Kip2, CDKN1C, KIP2

CDKN1C Antibody (N-term) western blot analysis in mouse kidney tissue lysates (35 ug/lane). This demonstrates the CDKN1C antibody detected the CDKN1C protein (arrow).

Fluorescent image of A549 cell stained with CDKN1C Antibody (N-term). A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CDKN1C primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml, 1 h at 37°C). CDKN1C immunoreactivity is localized to Nucleus significantly.

Quick Database Links

Gene Symbol

CDKN1C

UniProt

P49918

RefSeq (Protein):NP_001116103.1, NP_000067.1, NP_001116102.1