CDKN1A/p21CIP1 Rabbit pAb Discontinued

SKU: orb48324

This product can be custom made

Description

Rabbit polyclonal antibody to CDKN1A

Research Area

Epigenetics

Images & Validation

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Item 1 of 6

Tested Applications	ELISA, IF, IHC-P, WB
Dilution Range	WB: 1:500-2000, IHC-P: 1:50-200, IF/ICC: 1:20-100
Reactivity	Human, Mouse, Rat

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	A synthetic peptide corresponding to a sequence within amino acids 1-164 of human CDKN1A/p21CIP1 (NP_000380.1).
Molecular Weight	18kDa
Purification	Affinity purification
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Concentration	1 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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anti CAP20 antibody, anti CDKN1 antibody, anti CIP1 antibody, anti MDA-6 antibody, anti P21 antibody, anti SDI1 antibody, anti WAF1 antibody, anti p21CIP1 antibody

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Immunofluorescence analysis of C6 cells using CDKN1A/p21CIP1 Rabbit pAb (orb48324) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of HeLa cells using CDKN1A/p21CIP1 Rabbit pAb (orb48324) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using CDKN1A/p21CIP1 Rabbit pAb (orb48324) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunohistochemistry analysis of paraffin-embedded Human colon tissue using CDKN1A/p21CIP1 Rabbit pAb (orb48324) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Western blot analysis of lysates from MCF7 cells, using CDKN1A/p21CIP1 Rabbit pAb (orb48324) at 1:900 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Western blot analysis of various lysates, using CDKN1A/p21CIP1 Rabbit pAb (orb48324) at 1:900 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.