CDKN1A Antibody (C-term)

SKU: orb1936665

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cancer Biology, Cell Biology, Immunology & Inflammation

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Dilution Range: IF - 1:10-50, WB - 1:1000, IHC-P - 1:10-50
• Reactivity: Human, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD55404
• Immunogen: This CDKN1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 117-146 amino acids from the C-terminal region of human CDKN1A. Antigen Region: 117-146 aa.
• Target: CDKN1A (HGNC:1784)
• Molecular Weight: 18119 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Cyclin-dependent kinase inhibitor 1, CDK-interacting protein 1, Melanoma differentiation-associated protein 6, MDA-6, p21, CDKN1A, CAP20, CDKN1, CIP1, MDA6, PIC1, SDI1, WAF1

CDKN1A Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of CDKN1A Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

CDKN1A Antibody (C-term) western blot analysis in Jurkat cell line lysates (35 ug/lane). This demonstrates the CDKN1A antibody detected the CDKN1A protein (arrow).

CDKN1A Antibody (C-term) western blot analysis in mouse Neuro-2a cell line lysates (35 ug/lane). This demonstrates the CDKN1A antibody detected the CDKN1A protein (arrow).

Fluorescent image of A549 cell stained with CDKN1A Antibody (C-term). A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CDKN1A primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml, 1 h at 37°C). CDKN1A immunoreactivity is localized to Nucleus significantly.

Quick Database Links

Gene Symbol

CDKN1A (HGNC:1784)

UniProt

P38936

RefSeq (Protein):NP_000380.1, NP_001207707.1, NP_001207706.1, NP_510867.1