CDK9 Antibody

• Catalog Number: orb1259409
• Category: Antibodies
• Description: CDK9 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, IP, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 153-372 of human CDK9 (NP_001252.1).
• Concentration: batch dependent
• Dilution range: WB: 1:1000 - 1:3000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 40kDa
• Target: CDK9
• UniProt ID: P50750
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: CDK9, RP11-228B15.5, C-2k, CDC2L4, PITALRE, TAK, C
• Note: For research use only
• Application notes: WB: 1:1000 - 1:3000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using CDK9 antibody (orb1259409) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunofluorescence analysis of U2OS cells using CDK9 antibody (orb1259409). Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200 ug extracts of HeLa cells, using 3 ug CDK9 antibody (orb1259409). Western blot was performed from the immunoprecipitate using CDK9 antibody (orb1259409) at a dilution of 1:1000.