CDK9 Antibody

SKU: orb1259409

Description

CDK9 Antibody

Research Area

Cell Biology, Immunology & Inflammation, Signal Transduction

Images & Validation

• Tested Applications: IF, IHC, IP, WB
• Reactivity: Human, Mouse
• Application Notes:
  WB: 1:1000 - 1:3000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:100

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 153-372 of human CDK9 (NP_001252.1).
• Target: CDK9
• Molecular Weight: Observed: 40kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CDK9, RP11-228B15.5, C-2k, CDC2L4, PITALRE, TAK, CTK1

Western blot analysis of extracts of various cell lines, using CDK9 antibody (orb1259409) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunofluorescence analysis of U2OS cells using CDK9 antibody (orb1259409). Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200 ug extracts of HeLa cells, using 3 ug CDK9 antibody (orb1259409). Western blot was performed from the immunoprecipitate using CDK9 antibody (orb1259409) at a dilution of 1:1000.

Quick Database Links

Gene Symbol

CDK9

UniProt

P50750