Cdk5 (phospho Tyr15) rabbit pAb

• Catalog Number: orb767313
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Cdk5 (phospho-Tyr15)
• Clonality: Polyclonal
• Tested applications: ELISA, IHC-P, WB
• Reactivity: Human, Monkey, Mouse, Rat
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human CDK5 around the phosphorylation site of Tyr15. AA range:1-50
• Concentration: 1 mg/ml
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
• Conjugation: Unconjugated
• MW: 33
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-CDK5 antibody, anti-CDKN5 antibody, anti-Cycl
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot analysis of HT29 + PMA cells using Phospho-Cdk5 (Y15) Polyclonal Antibody.

Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using CDK5 (Phospho-Tyr15) Antibody.

Western blot analysis of lysates from COS7 cells treated with EGF 200ng/ml 30' and 293 cells treated with H2O2 100u, 15mins, using CDK5 (Phospho-Tyr15) Antibody. The lane on the right is blocked with the phospho peptide.