Cdk5 (phospho Tyr15) rabbit pAb

SKU: orb767313

Description

Rabbit polyclonal antibody to Cdk5 (phospho-Tyr15)

Images & Validation

• Tested Applications: ELISA, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
• Reactivity: Human, Monkey, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human CDK5 around the phosphorylation site of Tyr15. AA range:1-50
• Molecular Weight: 33
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-CDK5 antibody, anti-CDKN5 antibody, anti-Cyclin-dependent kinase 5 antibody, anti-Cell division protein kinase 5 antibody, anti-Serine/threonine-protein kinase PSSALRE antibody, anti-Tau protein kinase II catalytic subunit antibody, anti-TPKII catalytic subunit antibody

Western Blot analysis of HT29 + PMA cells using Phospho-Cdk5 (Y15) Polyclonal Antibody.

Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using CDK5 (Phospho-Tyr15) Antibody.

Western blot analysis of lysates from COS7 cells treated with EGF 200ng/ml 30' and 293 cells treated with H2O2 100u, 15mins, using CDK5 (Phospho-Tyr15) Antibody. The lane on the right is blocked with the phospho peptide.