CDK5 Antibody (C-term)

SKU: orb1788137

Description

Purified Rabbit Polyclonal Antibody (Pab)

Images & Validation

• Tested Applications: FC, IF, WB
• Dilution Range: IF: 1:25, WB: 1:1000, FC: 1:25
• Reactivity: Human, Mouse
• Predicted Reactivity: Other, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: This CDK5 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 254-289 amino acids from the C-terminal region of human CDK5. Antigen Region: 254-289 aa.
• Target: CDK5 (HGNC:1774)
• Molecular Weight: 33304 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Cyclin-dependent kinase 5, Cell division protein kinase 5, Serine/threonine-protein kinase PSSALRE, Tau protein kinase II catalytic subunit, TPKII catalytic subunit, CDK5, CDKN5

Flow cytometric analysis of K562 cells using CDK5 Antibody (C-term) (green) compared to an isotype control of rabbit IgG (blue). Diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.

Fluorescent image of A549 cells stained with CDK5 Antibody (C-term). Diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).

All lanes: Anti-CDK5 Antibody (C-term) at 1/1000 dilution. Lane 1: A431 whole cell lysates. Lane 2: Hela whole cell lysates. Lane 3: K562 whole cell lysates. Lane 4: NIH/3T3 whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 32 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

CDK5 (HGNC:1774)

UniProt

Q00535