CDK3 Antibody (N-term Y19)

• Catalog Number: orb1929189
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB15488
• Tested applications: FC, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 35046 Da
• Target: This CDK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 4-38 amino acids from the N-terminal region of human CDK3.
• UniProt ID: Q00526
• NCBI: NP_001249.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Cyclin-dependent kinase 3, Cell division protein k
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of CDK3 (arrow) using rabbit polyclonal CDK3 Antibody (N-term Y19). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CDK3 gene (Lane 2).

Flow cytometric analysis of NCI-H292 cells using CDK3 Antibody (N-term Y19) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human lung carcinoma reacted with CDK3 Antibody (N-term Y19), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.