CDH11 Antibody

• Catalog Number: orb1249099
• Category: Antibodies
• Description: CDH11 Antibody
• Target: CDH11
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: C-ERRGHLRPSFHG
• UniProt ID: P55287
• Tested applications: ELISA, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:In transfected HEK293 transiently expressing full-length Human CDH11 (myc and DYKDDDDK tagged), a band of approx. 130kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody. Recommended concentration, 0.5-1ug/ml.Purchase the +ve control! The transfected lysate used to QC this product is available for sale. Please email [email protected] to request datasheet, pricing and delivery information.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CDH11, cadherin 11, type 2, OB-cadherin (osteoblas
• Note: For research use only
• NCBI: NP_001788.2

HEK293 lysate (10 ug protein in RIPA buffer) overexpressing Human CDH11 with C-terminal MYC tag probed with orb1249099 (1 ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with orb1249099 (1 mg/ml) in Lane B.