CDH1 Antibody

SKU: orb1927420

Description

Mouse Monoclonal Antibody (Mab)

Research Area

Epigenetics & Chromatin

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB - 1:4000, IHC-P - 1:25
• Reactivity: Human, Mouse

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone No.: B035EV33X3X5
• Immunogen: Purified His-tagged CDH1 protein was used to produced this monoclonal antibody.
• Target: CDH1 (HGNC:1748)
• Molecular Weight: 97456 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Cadherin-1, CAM 120/80, Epithelial cadherin, E-cadherin, Uvomorulin, CD324, E-Cad/CTF1, E-Cad/CTF2, E-Cad/CTF3, CDH1, CDHE, UVO

All lanes: Anti-CDH1 at 1:4000 dilution. Lane 1: SW480 whole cell lysate. Lane 2: MCF-7 whole cell lysate. Lane 3: WiDr whole cell lysate. Lane 4: Mouse stomach lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 98 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Staining CDH1 in human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Quick Database Links

Gene Symbol

CDH1 (HGNC:1748)

UniProt

P12830

RefSeq (Protein):NP_004351.1