CDH1 Antibody

SKU: orb1788110

Description

Mouse Monoclonal Antibody (Mab)

Research Area

Signal Transduction

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB: 1:1000, IHC-P: 1:25
• Reactivity: Human, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1,k
• Immunogen: Purified His-tagged CDH1 protein was used to produced this monoclonal antibody. Antigen Region: 292-587 aa.
• Target: CDH1 (HGNC:1748)
• Molecular Weight: 97456 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Cadherin-1, CAM 120/80, Epithelial cadherin, E-cadherin, Uvomorulin, CD324, E-Cad/CTF1, E-Cad/CTF2, E-Cad/CTF3, CDH1, CDHE, UVO

Immunohistochemical analysis of paraffin-embedded H. stomach section using CDH1 Antibody. Diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

All lanes: Anti-CDH1 Antibody at 1:1000 dilution. Lane 1: MDA-MB-468 whole cell lysates. Lane 2: T47D whole cell lysates. Lane 3: MCF-7 whole cell lysates. Lane 4: WiDr whole cell lysates. Lane 5: mouse stomach lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 98 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

CDH1 (HGNC:1748)

UniProt

P12830

RefSeq (Protein):NP_004351.1