CDH1 Antibody

SKU: orb1246840

Description

CDH1 Antibody

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human, Mouse, Porcine, Rat
• Predicted Reactivity: Canine
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 85kDa band observed in Human Kidney lysates and in Pig Colon, Kidney and Lung lysates and ~90kDa in Mouse and Rat Lung lysates (calculated MW of 97.5kDa according to NP_004351.1). Recommended concentration: 0.3-1µg/mL. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Thyroid. Recommended concentration: 5µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-DYKINLKLMDNQNK
• Target: CDH1
• Molecular Weight: Approx 85 kDa
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CDH1, cadherin 1, type 1, E-cadherin (epithelial), Arc-1, CD324, CDHE, ECAD, LCAM, UVO, CAM 120/80, E-Cadherin, cadherin 1, E-cadherin (epithelial), cadherin-1, calcium-dependent adhesion protein, epithelial, cell-CAM 120/80, epithelial cadherin, uvomorulin

orb1246840 (0.5 ug/ml) staining of Human Kidney lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1246840 (0.3 ug/ml) staining of Pig Kidney (A) and Colon (B) lysates (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1246840 (0.3 ug/ml) staining of Mouse Lung (A) and Rat Lung (B) lysates (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1246840 (5 ug/ml) staining of paraffin embedded Human Thyroid. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

CDH1

UniProt

P12830

RefSeq (Protein):NP_004351.1