CDC42BPA Antibody

SKU: orb1247685

Description

CDC42BPA Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, IHC
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave an approx 30kDa and 40kDa bands in Human Pancreas lysates at 1µg/mL. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated sizes of 186kDa according to NP_055641.3 and 196kDa according to NP_003598.2. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Cerebellum shows cytoplasm staining in purkinje cells. Recommended concentration, 3-6µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: SGEVRLRQLEQFI-C
• Target: CDC42BPA
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CDC42BPA, MRCK, MRCKA, PK428, FLJ23347, KIAA0451, CDC42 binding protein kinase alpha (DMPK-like), ser-thr protein kinase PK428, myotonic dystrophy kinase-related CDC42-binding protein kinase alpha, Ser-Thr protein kinase related to the myotonic dystrophy protein kinase, DKFZp686L1738, CDC42 binding protein kinase beta

orb1247685 (3.8 ug/ml) staining of paraffin embedded Human Cerebellum Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

CDC42BPA

UniProt

Q5VT25

RefSeq (Protein):NP_055641.3, NP_003598.2