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Item 1 of 4
CDC37 Antibody (Center)
Catalog Number: orb1928181
| Catalog Number | orb1928181 |
|---|---|
| Category | Antibodies |
| Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB22954 |
| Tested applications | FC, IHC-P, WB |
| Reactivity | Human, Rat |
| Isotype | Rabbit IgG |
| Antibody Type | Primary Antibody |
| Dilution range | WB: 1:1000, WB: 1:2000, IHC-P: 1:50~100, FC: 1:10~50 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 44468 Da |
| Target | This CDC37 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 116-144 amino acids from the Central region of human CDC37. |
| UniProt ID | Q16543 |
| NCBI | NP_008996.1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Hsp90 co-chaperone Cdc37, Hsp90 chaperone protein Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Western blot analysis of CDC37 Antibody (Center) in MCF-7, A2058 cell line lysates (35 ug/lane). CDC37 (arrow) was detected using the purified Pab.
CDC37 Antibody (Center) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
CDC37 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded skin followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CDC37 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
All lanes: Anti-CDC37 Antibody (Center) at 1:2000 dilution. Lane 1: A431 whole cell lysate. Lane 2: C6 whole cell lysate. Lane 3: Jurkat whole cell lysate. Lane 4: K562 whole cell lysate. Lane 5: LNCaP whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 44 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
