CDC37 Antibody: Biotin

• Catalog Number: orb151598
• Category: Antibodies
• Description: Rabbit polyclonal to CDC37 (Biotin). Hsp90 co-chaperone Cdc37 is a protein that is encoded by the CDC37 gene. It has been found to form complexes with Hsp90 and a variety of protein kinases including CDK4, CDK6, SRC, RAF1, MOK and elF-2 alpha kinases. It is thought to play a critical role in directing Hsp90 to its target kinases. CDC37 is necessary for maintaining prostate tumor cell growth and represents a novel target in the exploration for multi-targeted therapies.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, IHC, WB
• Reactivity: Human
• Immunogen: Native human Cdc37, full length
• Concentration: 1.68 mg/ml
• Dilution range: WB (1:2000), ICC/IF (1:200)
• Conjugation: Biotin
• MW: 44.5kDa
• Target: CDC37
• Entrez: 11140
• UniProt ID: Q16543
• NCBI: NP_008996.1
• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 136.36mM Ethanolamine, and 9.55mM Sodium Bicarbonate in 95.45% PBS
• Alternative names: Hsp90 co chaperone Cdc37 antibody, CDC 37 antibody
• Note: For research use only
• Application notes: A 1:2000 dilution of SPC-142 was sufficient for detection of cdc37 in 20 µg of HeLa cell lysate by ECL immunoblot analysis.
• Expiration Date: 12 months from date of receipt.

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-CDC37 Polyclonal Antibody. Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-CDC37 Polyclonal Antibody at 1:200 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-CDC37 Antibody. (C) Composite. Heat Shocked at 42°C for 30 min.

Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of CDC37 protein using Rabbit Anti-CDC37 Polyclonal Antibody. Primary Antibody: Rabbit Anti-CDC37 Polyclonal Antibody at 1:2000.

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-CDC37 Polyclonal Antibody. Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-CDC37 Polyclonal Antibody at 1:200 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-CDC37 Antibody. (C) Composite. Heat Shocked at 42°C for 30 min.