CDC20 Antibody

SKU: orb1252791

Description

CDC20 Antibody

Images & Validation

• Tested Applications: FC, IF, IHC, WB
• Reactivity: Human
• Application Notes:
  Flow Cytometry: 0.5-1 ug/million cellsIF: 0.5-1 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Cdc20 antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone No.: AR12
• Immunogen: Denatured recombinant human protein was used as the immunogen for this Cdc20 antibody.
• Target: CDC20
• Purification: Protein G purified antibody

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Disclaimer: For research use only

Alternative Names

Cell division cycle protein 20 homolog, p55CDC, CDC20

IHC testing of FFPE gastric carcinoma with Cdc20 antibody (clone AR12)

SDS-PAGE Analysis of Purified, BSA-Free Cdc20 Antibody (clone AR12). Confirmation of Integrity and Purity of the Antibody.

Quick Database Links

Gene Symbol

CDC20

UniProt

Q12834