Cdc2 (phospho Tyr15) rabbit pAb

• Catalog Number: orb764156
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Cdc2 (phospho-Tyr15)
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human, Monkey, Mouse, Rat
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human CDC2 around the phosphorylation site of Tyr15. AA range:5-54
• Concentration: 1 mg/ml
• Dilution range: Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
• Conjugation: Unconjugated
• MW: 34
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-CDK1 antibody, anti-CDC2 antibody, anti-CDC28
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of lysates from Mouse-brain, Rat-brain, Hela cells treated with nocodazole 1ug/ml 18h, using CDC2 p-Thr15 Antibody. Primary Antibody was diluted at 1:1000 4°C over night, secondary antibody was diluted at 1:10000, 37° 1hour.

Western blot analysis of Cdc2 0.1ug/ml 18hour, 4°C over night, secondary antibody was diluted at 1:10000, 37° 1hour.

Western Blot analysis of various cells using Phospho-Cdc2 (Y15) Polyclonal Antibody.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using CDC2 (Phospho-Tyr15) Antibody.