CD99 Antibody Cocktail

• Catalog Number: orb317190
• Category: Antibodies
• Description: Recognizes a sialoglycoprotein of 27-32kDa, identified as CD99, or MIC2 gene product, or E2 antigen. MIC2 gene is located in the pseudo-autosomal region of the human X and Y chromosome. MIC2 gene encodes two distinct proteins, which are produced by alternative splicing of the CD99 gene transcript and are identified as bands of 30 and 32kDa (p30/32). Although its function is not fully understood, CD99 is implicated in various cellular processes including homotypic aggregation of T cells, upregulation of T cell receptor and MHS molecules, apoptosis of immature thymocytes and leukocyte diapedesis. CD99 is expressed on the cell membrane of some lymphocytes, cortical thymocytes, and granulosa cells of the ovary. Most pancreatic islet cells, Sertoli cells of the testis, and some endothelial cells express this antigen. Mature granulocytes express very little or no CD99. MIC2 is strongly expressed on Ewing's sarcoma cells and primitive peripheral neuroectodermal tumors.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 12E7 + MIC2/877
• Tested applications: FACS, IF, IHC-P
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Human acute lymphocytic leukemia T-cells (12E7) and recombinant human protein (MIC2/877) were used as the immunogen for the CD99 antibody cocktail.
• Dilution range: Flow cytometry: 5-10ul/million cells,Immunofluorescence: 1:100-1:200,Immunohistochemistry (FFPE): 1:100-1:200 for 30 min at RT
• Purity: Unpurified high titer supernatant
• Conjugation: Other
• Formula: Bioreactor concentrate with 0.05% sodium azide
• Hazard Information: This CD99 antibody cocktail is available for research use only.
• UniProt ID: P14209
• Storage: Store the CD99 antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: Bioreactor concentrate with 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the CD99 antibody cocktail should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC staining of human Ewing's sarcoma using CD99 Cocktail antibody