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CD86 Antibody

Catalog Number: orb606385

DispatchUsually dispatched within 5-10 working days
$ 410.00
Catalog Numberorb606385
CategoryAntibodies
DescriptionRecognizes a protein of 70kDa, which is identified as CD86. CD86 is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes. CD86 expression is rapidly upregulated by B cell specific stimuli with peak expression at 18 to 42 hours after stimulation. CD86, along with CD80/B71, is an important accessory molecule in T cell co-stimulation via its interaction with CD28 and CD152/CTLA4. Since CD86 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response. It is also found on malignant Hodgkin and Reed Sternberg (HRS) cells in Hodgkin's disease.
Species/HostRabbit
ClonalityRecombinant
Clone NumberC86/2160R
Tested applicationsELISA, FACS, IF, IHC-P, WB
ReactivityHuman, Mouse, Rat
IsotypeRabbit IgG, kappa
ImmunogenRecombinant human full-length protein was used as the immunogen for the recombinant CD86 antibody.
Antibody TypePrimary Antibody
Dilution rangeELISA: 2-4ug/ml (order BSA/azide-free format),Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
PurityProtein A affinity chromatography
ConjugationUnconjugated
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Hazard InformationThis recombinant CD86 antibody is available for research use only.
UniProt IDP42081
StorageStore the recombinant CD86 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Buffer/Preservatives0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
NoteFor research use only
Application notesOptimal dilution of the recombinant CD86 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Expiration Date12 months from date of receipt.
CD86 Antibody

IHC testing of FFPE human colon with recombinant CD86 antibody (C86/2160R). Required HIER: boil tissue sections in 10mM Tris buffer with 1mM EDTA, pH9.0, for 10-20 min followed by cooling at RT for 20 min.

CD86 Antibody

IF/ICC staining of human Raji cells with recombinant CD86 antibody (green) and Reddot nuclear stain (red).

CD86 Antibody

Western blot testing of human Ramos cell lysate with recombinant CD86 antibody. Expected molecular weight: 38-70 kDa depending on glycosylation level.

CD86 Antibody

Flow cytometry testing of human Ramos cells with recombinant CD86 antibody (blue, clone C86/2160R) and isotype control (red).

CD86 Antibody

SDS-PAGE analysis of purified, BSA-free recombinant CD86 antibody (clone C86/2160R) as confirmation of integrity and purity.

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