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Description
Research Area
Immunology & Inflammation
Images & Validation
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Item 1 of 1
| Tested Applications | WB |
|---|---|
| Application Notes |
Key Properties
−| Source | HEK293 cells |
|---|---|
| Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized Human B7-1, His Tag at 20ug/mL (100 ul/well),can bind Human CD28, Fc Tag with a linear of 0.2-20 ug/mL. Measured by its ability to induce IL2 secretion by Jurkat human acute T cell leukemia cells. The ED50 for this effect is typically 0.01 - 0.1 ug/mL in the presence of PHA. |
| Target | CD80 |
| Tag | His Tag |
| MW | 25.8 kDa |
| Purity | >90% as determined by SDS-PAGE.Less than 1.0 EU per ug by the LAL method. |
| Protein Sequence | Val 35 - Asn 242 |
Storage & Handling
−| Storage | Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | PBS, pH 7.4 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−CD80, B7, B7-1, B7.1, BB1, CD28LG, CD28LG1, LAB7
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Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
WB
Western Blot (IB, immunoblot)
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