CD74 Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb1816735
• Category: Antibodies
• Description: CD74 Recombinant Rabbit Monoclonal Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: E9C4
• Tested applications: FC, ICC, IF, IHC-Fr, IHC-P, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human CD74
• Antibody Type: Recombinant Antibody
• Concentration: 1mg/ml
• Dilution range: IHC-P=1:100-500, Flow-Cyt=1:50, WB=1:500-2000, IHC-F=1:100-500, IF=1:100-500, ICC/IF=1:50-200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 35 kDa
• Target: CD74
• UniProt ID: P04233
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS, Glycerol, rAlbumin.
• Alternative names: CD 74; CD74 antigen; CD74 molecule; CLIP; DHLAG; g
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (human spleen), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (CD74) Monoclonal Antibody, Unconjugated (orb1816735) at 1:1000 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human tonsil), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (CD74) Monoclonal Antibody, Unconjugated (orb1816735) at 1:1000 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.