CD74 / CLIP Antibody

• Catalog Number: orb750163
• Category: Antibodies
• Description: CD74 associates with class II major histocompatibility complex (MHC) and is an important chaperone that regulates antigen presentation for immune response. It also serves as cell surface receptor for the cytokine macrophage migration inhibitory factor (MIF) which, when bound to the encoded protein, initiates survival pathways and cell proliferation. This protein also interacts with amyloid precursor protein (APP) and suppresses the production of amyloid beta (Abeta). Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [RefSeq]
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: Recombinant full-length human protein was used as the immunogen for the CD74 antibody. The epitope is known to be extracellular.
• UniProt ID: P04233
• Tested applications: FACS, IF, IHC
• Dilution range: Flow Cytometry: 0.5-1ug/million cells in 0.1ml,Immunofluorescence: 1-2ug/ml,Immunohistochemistry: 1-2ug/ml,Prediluted IHC only format: incubate for 30 min at RT (1)
• Application notes: Titering of the CD74 antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Antibody Type: Primary Antibody
• Clone Number: CDLA74-1
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Research Area: Epigenetics, Immunology
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry testing of human Raji cells with CD74 antibody (clone CDLA74-1); Red = isotype control, Blue = CD74 antibody.

Immunofluorescent staining of human Raji cells with CD74 antibody (green, clone CDLA74-1) and Reddot nuclear stain (red).

IHC testing of FFPE human tonsil with CD74 antibody (clone CDLA74-1). HIER: FFPE testing requires sections to be boiled in pH6 10 mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.