CD63 Recombinant Rabbit Monoclonal Antibody

SKU: orb1151946

Description

CD63 Recombinant Rabbit Monoclonal Antibody

Research Area

Cancer Research, Cardiovascular Research, Cell Adhesion, Cell Biology, Tumor Biology

Images & Validation

• Tested Applications: ICC, IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:1000-5000, IHC-P=1:200-800, IHC-F=1:200-800, ICC/IF=1:50-200, IF=1:200-800
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: IgG
• Clone No.: 4C2
• Immunogen: A synthesized peptide derived from human CD63 (100-200aa)
• Target: CD63
• Molecular Weight: 30-50 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Lysosomal associated membrane protein 3; CD63 antigen; CD63 antigen melanoma 1 antigen; CD63 molecule; granulophysin; LAMP 3; LAMP3; lysosome associated membrane glycoprotein 3; CD 63; CD63 antigen(melanoma 1 antigen); CD63_HUMAN; gp55; granulophysin; LAMP-3; LIMP; Lysosomal-associated membrane protein 3; Melanoma-associated antigen ME491; NGA; Ocular melanoma-associated antigen; PTLGP40; Tetraspanin-30; Tspan-30; Mast cell antigen AD1; ME491; melanoma 1 antigen; Melanoma associated antigen ME491; Melanoma associated antigen MLA1; MGC72893; MLA 1; MLA1; ocular melanoma associated antigen; OMA81H; Tetraspanin 30; Tspan 30; TSPAN30; Tumor biomarkers; Platelets markers.

Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-CD63 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD63 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD63 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Quick Database Links

Gene Symbol

CD63

UniProt

P08962