CD63 Antibody / LAMP-3

• Catalog Number: orb1151816
• Category: Antibodies
• Description: Mouse monoclonal antibody to CD63
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: LAMP3/4949
• Tested applications: ELISA, FACS, IF, IHC-P
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Full-length human CD63 protein was used as the immunogen for the CD63 antibody.
• Antibody Type: Primary Antibody
• Dilution range: ELISA: 1-5ug/ml (order BSA-free format for coating),Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This CD63 antibody is available for research use only.
• UniProt ID: P08962
• Storage: Aliquot the CD63 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the CD63 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

FACS staining of bead-bound exosomes derived from MCF-7 cells: Unstained exosomes (gray) and exosomes stained with CD63 antibody (orange, clone LAMP3/4949).

FACS staining of human MCF-7 cells with CD63 antibody (blue, clone LAMP3/4949), unstained cells (blue, clone gray).

IHC staining of FFPE human tonsil tissue with CD63 antibody (clone LAMP3/4949) at 1 ug/ml. Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free CD63 antibody (clone LAMP3/4949) as confirmation of integrity and purity.