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Catalog Number | orb749523 |
---|---|
Category | Antibodies |
Description | Anti-CD57 marks a subset of lymphocytes known as natural killer (NK) cells. Follicular center cell lymphomas often contain many NK cells within the neoplastic follicles. Anti-CD57 also stains neuroendocrine cells and their derived tumors, including carcinoid tumor and medulloblastoma. Anti-CD57 can also be useful in separating type B3 thymoma from thymic carcinoma when combined with a panel that includes antibodies against GLUT1, CD5, and CEA. |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | HNK-1 + NK-1 |
Tested applications | IF, IHC-P |
Reactivity | Human |
Isotype | Mouse IgM, kappa |
Immunogen | Membrane antigen from HSB-2 cells was used as the immunogen for the CD57 antibody. |
Dilution range | Immunofluorescence: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-4ug/ml for 30 min at RT |
Purity | PEG precipitation |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide |
Hazard Information | This CD57 antibody cocktail is available for research use only. |
UniProt ID | Q9P2W7 |
Storage | Store the CD57 antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide). |
Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide |
Note | For research use only |
Application notes | Optimal dilution of the CD57 antibody cocktail should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Expiration Date | 12 months from date of receipt. |
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