ICAM3 Antibody

SKU: orb1252757

Description

ICAM3 Antibody

Research Area

Neuroscience

Images & Validation

• Tested Applications: Blocking, FC, IF, IHC
• Reactivity: Human
• Application Notes:
  Functional Activity (Blocking): Blocks CD50-CD11a interactionFlow Cytometry: 0.5-1 ug/million cellsIF: 0.5-1 µg/mLIHC (FFPE): 0.5-1 µg/mL for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD50 antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b, kappa
• Clone No.: 186-2G9
• Immunogen: Stimulated human leukocytes were used as the immunogen for this CD50 antibody.
• Target: ICAM3
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Intercellular adhesion molecule 3, ICAM-3, CDw50, ICAM-R, CD50, ICAM3

IHC analysis of FFPE human tonsil with CD50 antibody (clone 186-2G9).

Quick Database Links

Gene Symbol

ICAM3

UniProt

P32942