CD47 Antibody / IAP / Integrin Associated Protein

• Catalog Number: orb749906
• Category: Antibodies
• Description: This antibody reacts with Ig domain of CD47 protein. CD47, originally named integrin-associated protein (IAP), is a 50kDa protein containing five membrane-spanning sequences and a short cytoplasmic tail. CD47 plays a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It is important in memory formation and synaptic plasticity in the hippocampus. CD47 may play a role in membrane transport and/or integrin dependent signal transduction.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: Recombinant human protein was used as the immunogen for the CD47 antibody.
• UniProt ID: Q08722
• Tested applications: FACS, IF
• Dilution range: Immunofluorescence: 1-2ug/ml,Flow cytometry: 1-2ug/million cells
• Application notes: Optimal dilution of the HCAM antibody should be determined by the researcher.1. Staining of formalin/paraffin tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Antibody Type: Primary Antibody
• Clone Number: IAP/964
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This CD47 antibody is available for research use only.
• Note: For research use only

Immunofluorescent staining of live human Jurkat cells with CD47 antibody (green, clone IAP/964) and Reddot nuclear stain (red).

Immunofluorescent staining of PFA-fixed human MCF7 cells with CD47 antibody (green, clone IAP/964) and Reddot nuclear stain (red).

Flow cytometry staining of live human Jukat cells with CD47 antibody (clone IAP/964); Red = isotype control, Blue = CD47 antibody.

SDS-PAGE analysis of purified, BSA-free CD47 antibody (clone IAP/964) as confirmation of integrity and purity.