CD47 Antibody / IAP / Integrin Associated Protein

• Catalog Number: orb1151572
• Category: Antibodies
• Description: Rabbit monoclonal antibody to CD47
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: Rabbit IgG, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: A portion of amino acids 18-135 from the human protein was used as the immunogen for the recombinant CD47 antibody.
• UniProt ID: Q08722
• Tested applications: ELISA, IHC-P
• Dilution range: ELISA (order BSA-free format for coating),Immunohistochemistry (FFPE): 1-2ug/ml
• Application notes: Optimal dilution of the recombinant CD47 antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: CD47/6362R
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This recombinant CD47 antibody is available for research use only.
• Note: For research use only

IHC staining of FFPE human prostate carcinoma tissue with recombinant CD47 antibody (clone CD47/6362R). Negative control inset: PBS used instead of primary antibody to control for secondary Ab binding. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human prostate carcinoma tissue with recombinant CD47 antibody (clone CD47/6362R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant CD47 antibody (clone CD47/6362R) as confirmation of integrity and purity.