CD45/LCA Antibody

SKU: orb1534844

Description

CD45/LCA Antibody

Images & Validation

• Tested Applications: IHC, IHC-P
• Dilution Range: IHC, IHC-P (2.5 - 5 µg/ml)
• Reactivity: Human
• Application Notes:
  Further information: Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 2.5-5 ug/ml.

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic 16 amino acid peptide from internal region of human CD45. Percent identity with other species by BLAST analysis: Human, Gorilla (100%); Gibbon (88%).
• Target: CD45 / LCA
• Purification: Immunoaffinity purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS, 0.1% Sodium Azide
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PTPRC, B220, CD45R, CD45, gp180, Leukocyte common antigen, L-CA, Leucocyte common antigen, LY5, T200, T200 glycoprotein, T200 leukocyte common antigen, CD45 antigen, LCA

Anti-CD45 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 2.5-5 ug/ml.

Quick Database Links

Gene Symbol

CD45 / LCA